Design and analysis of jammable granular systems

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2013.Cataloged from PDF version of thesis.Includes bibliographical references (p. 102-110).Jamming--the mechanism by which granular media can transition between liquid-like and solid-like states-has recently been demonstrated as a variable strength and stiffness mechanism in a range of applications. As a low-cost and simple means for achieving tunable mechanical properties, jamming has been used in systems ranging from architectural to medical ones. This thesis explores the utility of jamming for robotic manipulation applications, both at a fundamental level of understanding how granular properties affect the performance of jammed systems, and at a more applied level of designing functional robotic components. Specifically, the purpose of this thesis was to enable engineers to design jammable robotic systems in a principled manner. Three parallel yet related studies were conducted to work towards this goal. First, an experimental analysis was conducted to determine whether the bulk shear strength of granular systems can be correlated with grain properties-such as ones concerning shape, size distribution, and surface texture-extracted from 2D silhouettes of grains. Second, a novel medium composed of a mixture of hard and soft spheres was proposed to achieve variable strength and stiffness properties as a function of confining pressure; experimental analysis was conducted on this system with not only varying confining pressures but also varying mixing ratios of hard and soft spheres. Finally, the design and analysis of a novel jammable robotic manipulator-with the goal of maximizing both the strength and articulation of the system-is presented.by Nadia G. Cheng.Ph.D

Design and analysis of active fluid-and-cellular solid composites for controllable stiffness robotic elements

Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2009.Includes bibliographical references (leaves 107-108).The purpose of this thesis is to investigate the use of a new class of materials for realizing soft robots. Specifically, meso-scale composites--composed of cellular solids impregnated with active fluids-were be designed to have controllable stiffness to take the form of a continuous body of a soft robot. This represents an improvement compared to past efforts in soft robotics, which often involved modifying the infrastructure of current, rigid robots to yield softer ones. This latter approach often faced the challenges of developing actuators that were "soft" but still discrete, and were limited in performance. In contrast, the controllable-stiffness composites proposed in this thesis eliminate the need for multiple actuators; a single structure can transition between various states to serve as both rigid, load-bearing components as well as morphable, compliant ones. While the vast range of fluid-foam combinations for such an application have yet to be explored, the work presented here focuses on a specific composite: open-cell polyurethane foam impregnated with wax. This type of composite can be thermally activated to exhibit both solid and nearly fluid states (while the wax can be melted to become a fluid, the foam holds the composite together as a pseudo-solid). This thesis discusses the research that has been conducted to 1) characterize the mechanical properties of wax-foam composites as well as 2) investigate possible ways in which the composites can be used as robotic components.by Nadia G. Cheng.S.M

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field